..Oxford slices through this Gordian knot by reading bases in triples (who ever heard of anything useful coming from reading bases in threes?).

... Not many companies get big bucks for mate pair reagents, but that's definitely a technology that isn't long for this world.

ComSeq is a set of utilities used for simulating and analyzing compressed-sensing pooling design experiments for next-generation sequencing projects. The aim is to identify novel rare alleles, or detect individuals who are carriers of known SNPs in a large population. The package enables an in-silico simulation of a sequencing pooling experiments, including modeling of sequencing error parameters. The package also enables reconstructing genotypes of individuals profiled in real experiments. Detailed instructions and usage examples are given in the README file.

RT @BioInfo: Google Maps Compilation of NGS instruments

novel approach for using massively parallel short-read sequencing to generate fast and inexpensive de novo genomic assemblies comparable to those generated by capillary-based methods.